Solution stirring initiates nucleation and improves the quality of adenosine deaminase crystals

Acta Crystallogr D Biol Crystallogr. 2005 Jun;61(Pt 6):759-62. doi: 10.1107/S0907444905013466. Epub 2005 May 26.


Crystals of bovine adenosine deaminase (ADA) grown over a two-week period in the presence of an inhibitor (ADA complex) were found to be of low quality for X-ray diffraction analysis. Furthermore, ADA incubated in the absence of an inhibitor (ADA native) did not form any crystals using conventional crystallization methods. A solution-stirring technique was used to obtain high-quality ADA complex and ADA native crystals. The crystals obtained using this technique were found to be of high quality and were shown to have high structural resolution for X-ray diffraction analyses. The results reported here indicate that the solution-stirring technique promotes nucleation and improves the quality of protein crystals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Deaminase / chemistry*
  • Animals
  • Cattle
  • Crystallization
  • Crystallography, X-Ray / methods*
  • Solutions / chemistry


  • Solutions
  • Adenosine Deaminase