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Review
, 61 (Pt 6), 793-8

Protein Crystal Perfection and Its Application

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Review

Protein Crystal Perfection and Its Application

John R Helliwell. Acta Crystallogr D Biol Crystallogr.

Abstract

Knowledge of protein crystal perfection in theory and practice is reviewed. X-ray methods of assessing perfection such as mosaicity, topography and reciprocal-space mapping are described. X-ray diffraction physics applications of protein crystals such as in Laue geometry, the large-angle oscillation technique and forming polychromatic profiles across diffraction spots are covered. Cryo- and room-temperature cases are discussed including in X-ray and neutron protein crystallography. Experience of freezing very large crystals, which are commonly used today in neutron protein crystallography, is highlighted.

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