Region-specific changes of calcium/calmodulin-dependent protein kinase IV in the mouse brain following chronic morphine treatment

Neuroreport. 2005 Jun 21;16(9):879-82. doi: 10.1097/00001756-200506210-00001.

Abstract

In this study, we examined changes in expression of calcium/calmodulin-dependent protein kinase IV (CaMKIV) in the mouse brain following chronic morphine treatment. Double immunohistochemical staining showed strong colocalization of CaMKIV with mu-opioid receptors. Chronic treatment with morphine produced an increase in expression of CaMKIV and phosphorylated cAMP response element-binding protein (pCREB) in the CA3 region of the hippocampus, whereas a decrease in CaMKIV and pCREB expression was observed in the caudate putamen. Interestingly, chronic morphine induced a decrease in protein expression of CaMKIV in the basolateral amygdale and the primary somatosensory cortex without any concomitant changes in pCREB. These findings suggest that CaMKIV-dependent signaling may play a role in chronic morphine-induced neuroplasticity in a brain region-specific manner.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Brain / anatomy & histology
  • Brain / drug effects*
  • Brain / enzymology
  • Brain / metabolism
  • Calcium-Calmodulin-Dependent Protein Kinase Type 1
  • Calcium-Calmodulin-Dependent Protein Kinase Type 4
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
  • Cyclic AMP Response Element-Binding Protein / metabolism
  • Gene Expression Regulation / drug effects*
  • Immunohistochemistry / methods
  • Indoles / metabolism
  • Male
  • Mice
  • Morphine / administration & dosage*
  • Narcotics / administration & dosage*
  • Phosphorylation
  • Receptors, Opioid, mu / metabolism

Substances

  • Cyclic AMP Response Element-Binding Protein
  • Indoles
  • Narcotics
  • Receptors, Opioid, mu
  • DAPI
  • Morphine
  • Calcium-Calmodulin-Dependent Protein Kinase Type 1
  • Calcium-Calmodulin-Dependent Protein Kinase Type 4
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Camk1 protein, mouse
  • Camk4 protein, mouse
  • Pnck protein, mouse