Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
, 6 (6), 507-12

Gene Targeting in Mice: Functional Analysis of the Mammalian Genome for the Twenty-First Century

Affiliations
Review

Gene Targeting in Mice: Functional Analysis of the Mammalian Genome for the Twenty-First Century

Mario R Capecchi. Nat Rev Genet.

Abstract

Gene targeting in mouse embryonic stem cells has become the 'gold standard' for determining gene function in mammals. Since its inception, this technology has revolutionized the study of mammalian biology and human medicine. Here I provide a personal account of the work that led to the generation of gene targeting which now lies at the centre of functional genomic analysis.

Similar articles

  • Engineering Chromosomal Rearrangements in Mice
    Y Yu et al. Nat Rev Genet 2 (10), 780-90. PMID 11584294. - Review
    The combination of gene-targeting techniques in mouse embryonic stem cells and the Cre/loxP site-specific recombination system has resulted in the emergence of chromosoma …
  • Embryonic Stem Cells and Gene Targeting
    B Ledermann. Exp Physiol 85 (6), 603-13. PMID 11187956. - Review
    The development of gene targeting technology, the exchange of an endogenous allele of a target gene for a mutated copy via homologous recombination, and the application o …
  • Generation of PDE4 Knockout Mice by Gene Targeting
    SL Jin et al. Methods Mol Biol 307, 191-210. PMID 15988065.
    The development of gene-targeting techniques has ushered in a new era in mouse genetics. Two discoveries have been instrumental: the finding that an exogenous DNA introdu …
  • Manipulating Mammalian Genome by Gene Targeting
    S Volarević et al. Croat Med J 40 (3), 368-74. PMID 10411964. - Review
    The development of strategies which allow the inactivation of specific murine genes by homologous recombination in embryonic cells has revolutionized biological science i …
  • Genetic Engineering in the Mouse: Tuning TNF/TNFR Expression
    E Douni et al. Methods Mol Med 98, 137-70. PMID 15064438.
    With the exponential increase in the number of genes identified by various genome projects, it has become imperative that efficient methods be developed for deciphering g …
See all similar articles

Cited by 238 PubMed Central articles

See all "Cited by" articles

LinkOut - more resources

Feedback