C. elegans daf-6 encodes a patched-related protein required for lumen formation

Dev Cell. 2005 Jun;8(6):893-906. doi: 10.1016/j.devcel.2005.03.009.


Sensory organs are often composed of neuronal sensory endings accommodated in a lumen formed by ensheathing epithelia or glia. Here we show that lumen formation in the C. elegans amphid sensory organ requires the gene daf-6. daf-6 encodes a Patched-related protein that localizes to the luminal surfaces of the amphid channel and other C. elegans tubes. While daf-6 mutants display only amphid lumen defects, animals defective for both daf-6 and the Dispatched gene che-14 exhibit defects in all tubular structures that express daf-6. Furthermore, DAF-6 protein is mislocalized, and lumen morphogenesis is abnormal, in mutants with defective sensory neuron endings. We propose that amphid lumen morphogenesis is coordinated by neuron-derived cues and a DAF-6/CHE-14 system that regulates vesicle dynamics during tubulogenesis.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins / genetics*
  • Caenorhabditis elegans Proteins / metabolism
  • Fluorescent Antibody Technique / methods
  • Gene Expression Regulation, Developmental / physiology*
  • Green Fluorescent Proteins / metabolism
  • Heat-Shock Proteins / metabolism
  • Microscopy, Electron, Transmission / methods
  • Molecular Biology / methods
  • Molecular Sequence Data
  • Mutagenesis / physiology
  • Mutation / physiology
  • Phenotype
  • Recombinant Fusion Proteins
  • Sense Organs / embryology*
  • Sense Organs / metabolism*
  • Sense Organs / ultrastructure
  • Sequence Homology, Amino Acid
  • Subcellular Fractions / metabolism
  • Transformation, Genetic / physiology


  • Caenorhabditis elegans Proteins
  • DAF-6 protein, C elegans
  • Heat-Shock Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins