In vitro activity of the essential oil of Cinnamomum zeylanicum and eugenol in peroxynitrite-induced oxidative processes

J Agric Food Chem. 2005 Jun 15;53(12):4762-5. doi: 10.1021/jf050183e.


The essential oil obtained from the bark of Cinnamomum zeylanicum Blume (Lauraceae) and three of its main components, eugenol, (E)-cinnamaldehyde, and linalool (representing 82.5% of the total composition), were tested in two in vitro models of peroxynitrite-induced nitration and lipid peroxidation. The essential oil and eugenol showed very powerful activities, decreasing 3-nitrotyrosine formation with IC50 values of 18.4 microg/mL and 46.7 microM, respectively (reference compound, ascorbic acid, 71.3 microg/mL and 405.0 microM) and also inhibiting the peroxynitrite-induced lipid peroxidation showing an IC50 of 2.0 microg/mL and 13.1 microM, respectively, against 59.0 microg/mL (235.5 microM) of the reference compound Trolox. On the contrary, (E)-cinnamaldehyde and linalool were completely inactive.

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • Cinnamomum zeylanicum / chemistry*
  • Eugenol / pharmacology*
  • Lipid Peroxidation / drug effects
  • Microsomes, Liver / drug effects
  • Microsomes, Liver / metabolism
  • Nitrates / chemistry
  • Oils, Volatile / pharmacology*
  • Oxidation-Reduction
  • Peroxynitrous Acid / pharmacology*
  • Rats
  • Tyrosine / analogs & derivatives*
  • Tyrosine / chemistry


  • Nitrates
  • Oils, Volatile
  • Peroxynitrous Acid
  • 3-nitrotyrosine
  • Eugenol
  • Tyrosine