Novel application of layered expression scanning for proteomic profiling of plucked hair follicles

Dermatology. 2005;210(4):273-8. doi: 10.1159/000084750.


Background: There is a need for a technology that can quantitatively assay multiple proteins from a single hair follicle while preserving the morphology of the follicle. For proteomic profiling, the technology should be less labor intensive, with a higher throughput, more quantitative and more reproducible than immunohistochemistry.

Objective: To test the ability of a novel method, layered expression scanning of hair (LES-hair) to detect the levels and localization of proteins in plucked hair follicles.

Methods: LES-hair was used to assay proteins in the plucked hair follicle.

Results: LES-hair detected differential expression of proteins within discrete regions of the plucked hair follicle. These proteins included cleaved caspase 3, Ki-67 and the phosphorylated forms of c-Kit, epidermal growth factor receptor and vascular endothelial growth factor receptor.

Conclusion: LES-hair provides a research tool for studying the basic biology of plucked hair follicles and has potential clinical applications such as monitoring treatment of alopecia or using plucked hair follicles as a surrogate tissue to monitor pharmacodynamic effects of targeted cancer therapies.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Blotting, Western
  • Culture Techniques
  • ErbB Receptors / metabolism
  • ErbB Receptors / ultrastructure*
  • Gene Expression Regulation
  • Hair Diseases / diagnosis
  • Hair Diseases / genetics
  • Hair Follicle / ultrastructure*
  • Humans
  • Male
  • Microscopy, Electron, Scanning
  • Proteins / analysis
  • Proteins / genetics
  • Proteome / genetics
  • Proteome / ultrastructure*
  • Sampling Studies
  • Sensitivity and Specificity


  • Proteins
  • Proteome
  • ErbB Receptors