Regulatory effects of emodin on NF-kappaB activation and inflammatory cytokine expression in RAW 264.7 macrophages
- PMID: 15942676
Regulatory effects of emodin on NF-kappaB activation and inflammatory cytokine expression in RAW 264.7 macrophages
Abstract
Emodin, an anthraquinones component of Rheum palmatun, has been used for anti-inflammatory purposes. However, its underlying molecular effect(s) on target cells remain to be well clarified. Thus, our current study was aimed at investigating the regulatory mechanism of emodin on liposaccharide-induced inflammatory responses in RAW 264.7 macrophages by RT-PCR, Western blot analysis, immunocytochemical staining and immunofluorescence analysis. It was found that a treatment of 20 microg/ml emodin inhibited the expression of a panel of inflammatory-associated genes, including TNFalpha, iNOS, IL-10, cytosolic IkappaBalpha, IKK-alpha and IKK-gamma, to different extents as well as the nuclear translocation of NF-kappaB (nuclear factor-kappaB). The promoting effect of emodin on the production and translocation of p105 (the precursor of NF-kappaB p50) was time-dependent and reached a maximum at 5 h. Our data suggest that emodin plays its anti-inflammatory roles by regulating inflammatory cytokines, specifically by suppressing NF-kappaB activation.
Similar articles
-
p50 nuclear factor-kappaB overexpression in tumor-associated macrophages inhibits M1 inflammatory responses and antitumor resistance.Cancer Res. 2006 Dec 1;66(23):11432-40. doi: 10.1158/0008-5472.CAN-06-1867. Cancer Res. 2006. PMID: 17145890
-
Bis-(3-hydroxyphenyl) diselenide inhibits LPS-stimulated iNOS and COX-2 expression in RAW 264.7 macrophage cells through the NF-kappaB inactivation.J Pharm Pharmacol. 2009 Apr;61(4):479-86. doi: 10.1211/jpp/61.04.0010. J Pharm Pharmacol. 2009. PMID: 19298695
-
Suppressive effect of novel aromatic diamine compound on nuclear factor-kappaB-dependent expression of inducible nitric oxide synthase in macrophages.Eur J Pharmacol. 2005 Oct 3;521(1-3):1-8. doi: 10.1016/j.ejphar.2005.07.013. Epub 2005 Sep 23. Eur J Pharmacol. 2005. PMID: 16183054
-
[Advances in the study on molecular mechanism of diabetic nephropathy treated with Rheum officinale].Zhongguo Zhong Yao Za Zhi. 2003 Aug;28(8):703-5. Zhongguo Zhong Yao Za Zhi. 2003. PMID: 15224660 Review. Chinese. No abstract available.
-
Attenuation of tumor necrosis factor alpha gene transcription in macrophages by an autocrine factor.Cold Spring Harb Symp Quant Biol. 1999;64:437-44. doi: 10.1101/sqb.1999.64.437. Cold Spring Harb Symp Quant Biol. 1999. PMID: 11232319 Review. No abstract available.
Cited by
-
Emodin elicits cytotoxicity in human lung adenocarcinoma A549 cells through inducing apoptosis.Inflammopharmacology. 2014 Apr;22(2):127-34. doi: 10.1007/s10787-013-0186-4. Epub 2013 Aug 22. Inflammopharmacology. 2014. PMID: 23975033
-
Topical anti-inflammatory activity of Polygonum cuspidatum extract in the TPA model of mouse ear inflammation.J Inflamm (Lond). 2008 Feb 8;5:1. doi: 10.1186/1476-9255-5-1. J Inflamm (Lond). 2008. PMID: 18261214 Free PMC article.
-
Tetrahydroanthraquinone Derivative (±)-4-Deoxyaustrocortilutein Induces Cell Cycle Arrest and Apoptosis in Melanoma Cells via Upregulation of p21 and p53 and Downregulation of NF-kappaB.J Cancer. 2016 Mar 12;7(5):555-68. doi: 10.7150/jca.13614. eCollection 2016. J Cancer. 2016. PMID: 27053954 Free PMC article.
-
Emodin reverses gemcitabine resistance in pancreatic cancer cells via the mitochondrial apoptosis pathway in vitro.Int J Oncol. 2012 Apr;40(4):1049-57. doi: 10.3892/ijo.2011.1285. Epub 2011 Dec 7. Int J Oncol. 2012. PMID: 22159556 Free PMC article.
-
Emodin enhances alveolar epithelial barrier function in rats with experimental acute pancreatitis.World J Gastroenterol. 2010 Jun 28;16(24):2994-3001. doi: 10.3748/wjg.v16.i24.2994. World J Gastroenterol. 2010. PMID: 20572302 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
