[Mutation of p53 and overexpression of STK15 in laryngeal squamous-cell carcinoma]

Zhonghua Zhong Liu Za Zhi. 2005 Mar;27(3):134-7.
[Article in Chinese]

Abstract

Objective: To explore the relationship between p53 gene mutations and STK15 abnormal expression in the development of human laryngeal squamous-cell carcinoma (LSCC).

Methods: LSCC tissues and matched normal tissues were taken during operation from 55 patients without previous chemotherapy or radiotherapy. Following polymerase chain reaction amplification direct sequencing single strand conformational polymorphism (PCR-SSCP) combined with silver staining were used to detect mutations of p53 gene in exons 7 and 8 (p53E7 and p53E8) using genomic DNA from 110 specimens including 55 LSCC tissues and 55 matched normal tissues. STK15 expression were evaluated by RT-PCR with beta-actin as internal control.

Results: The mutation rate of p53E7 was 30.9% (compared to normal tissues, chi(2) = 8.66, P < 0.01). There was no mutation in p53E8. In 38 of the 55 cases (69.1%), the STK15 mRNA expression level was higher than that of the paired normal tissue. The STK15 to beta-actin ratio of average density value was 1.22 +/- 0.49 in the cancer tissue, and 0.99 +/- 0.54 in the normal tissues (t = 4.539, P < 0.01). In 14 of the 17 cases (82.4%) with p53E7 mutations, the STK15 expression was higher than that of normal tissue. In the 38 cases with STK15 over-expression, p53E7 mutation was found in 14 cases (36.8%). The rate of concurrence of p53E gene mutations and STK15 over-expression (25.5%) was higher than that of only p53E gene mutations (chi(2) = 26.025, P < 0.01).

Conclusion: There is significant association between p53 gene mutation and STK15 over-expression in laryngeal squamous-cell carcinoma.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Aurora Kinase A
  • Aurora Kinases
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / metabolism
  • Exons
  • Frameshift Mutation*
  • Gene Expression Regulation, Neoplastic
  • Genes, p53 / genetics*
  • Humans
  • Laryngeal Neoplasms / genetics*
  • Laryngeal Neoplasms / metabolism
  • Mutation, Missense
  • Protein-Serine-Threonine Kinases / biosynthesis*
  • Protein-Serine-Threonine Kinases / genetics
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics

Substances

  • Actins
  • RNA, Messenger
  • AURKA protein, human
  • Aurora Kinase A
  • Aurora Kinases
  • Protein-Serine-Threonine Kinases