The PhoP/PhoQ system controls the intramacrophage type three secretion system of Salmonella enterica

Mol Microbiol. 2005 Jul;57(1):85-96. doi: 10.1111/j.1365-2958.2005.04668.x.

Abstract

Spi/Ssa is a unique type three secretion system that functions exclusively when Salmonella enterica is inside eukaryotic cells. Expression of the Spi/Ssa system and its secreted effectors is dependent on SsrB/SpiR, a two-component regulatory system encoded in the SPI-2 pathogenicity island that also harbours the spi/ssa genes. Here we determine that the PhoP/PhoQ two-component system controls the intramacrophage expression of spi/ssa genes by regulating the SsrB/SpiR system. We establish that PhoP regulates transcription of the response regulator SsrB and demonstrate binding of the PhoP protein to the ssrB promoter both in vivo using chromatin immunoprecipitation in Salmonella-infected macrophages, and in vitro using purified PhoP protein. We show that PhoP controls the SpiR sensor post-transcriptionally and identify a region in the 5' untranslated region of the spiR message that is required for this effect. The demonstration that the PhoP/PhoQ system is directly involved in the regulation of the SPI-2 pathogenicity island highlights PhoP/PhoQ's central role in Salmonella virulence. We suggest that different regulatory systems convey distinct signals over time to produce the SsrB/SpiR system, which then modulates expression of the Spi/Ssa apparatus and secreted effectors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 5' Untranslated Regions
  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Base Sequence
  • Cells, Cultured
  • Chromatin Immunoprecipitation
  • Gene Expression Regulation, Bacterial*
  • Genomic Islands / genetics
  • Macrophages / microbiology
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Salmonella enterica / genetics
  • Salmonella enterica / metabolism
  • Salmonella enterica / pathogenicity*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Virulence / genetics

Substances

  • 5' Untranslated Regions
  • Bacterial Proteins
  • PhoQ protein, Bacteria
  • SpiC protein, Salmonella
  • SpiR protein, Salmonella enterica
  • SsrB protein, Salmonella typhimurium
  • Transcription Factors
  • PhoP protein, Bacteria