Protein fibers are fundamental building blocks of life playing an essential role in motility, elasticity, scaffolding, stabilization and the protection of cells, tissues and organisms. Despite nearly a century of research into the assembly mechanisms and structures of fibrous proteins, only limited information is still available. Within the past decade, however, insights have been provided into how some fibrous proteins assemble and how they function in biology. In addition, efforts are increasingly being made to employ protein fibers as performance molecules in man-made medical and technical applications.