Shaping time: chromatin structure and the DNA replication programme

Trends Genet. 2005 Aug;21(8):444-9. doi: 10.1016/j.tig.2005.05.012.


DNA is replicated according to a precise and reproducible temporal pattern. The S-phase programme has previously been analyzed in metazoan and yeast cells using different methods: cytological chromosome banding in human cells and DNA isotopic-labeling techniques in yeast. Microarray-based approaches for the analysis of the replication programme and chromatin structure are bringing us closer to a molecular understanding of the factors that determine replication time. In this article, I assess the impact of recent investigations and compare our knowledge of DNA replication-timing controls in yeast with those of metazoans.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Chromatin / metabolism*
  • DNA Replication*
  • DNA, Fungal / biosynthesis
  • DNA, Fungal / genetics
  • Genome, Fungal
  • Genome, Human
  • Humans
  • Kinetics
  • Oligonucleotide Array Sequence Analysis
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Species Specificity


  • Chromatin
  • DNA, Fungal