Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome

J Proteome Res. May-Jun 2005;4(3):958-66. doi: 10.1021/pr050036w.

Abstract

Schistosoma mansoni is a parasitic worm that lives in the blood vessels of its host. We mapped the S. mansoni tegumental outer-surface structure proteome by 1D SDS-PAGE and LC-MS/MS and an EST-database from the ongoing genome-sequencing project. We identified 740 proteins of which 43 were tegument-specific. Many of these proteins show no homology to any nonschistosomal protein, demonstrating that the schistosomal outer-surface comprises specific and unique proteins, likely to be critical for parasite survival.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • Computational Biology
  • Electrophoresis, Polyacrylamide Gel
  • Helminth Proteins / analysis*
  • Helminth Proteins / physiology
  • Mass Spectrometry
  • Proteome / analysis
  • Proteomics / methods*
  • Schistosoma mansoni / chemistry*

Substances

  • Helminth Proteins
  • Proteome