Abstract
The steroid/sterol sulfotransferase gene (SULT2B1) encodes for two isozymes of which one (SULT2B1b) sulfonates cholesterol and is selectively expressed in skin. The human SULT2B1 gene contains neither a TATAAA nor a CCAAT motif upstream of the coding region for SULT2B1b; however, this area is GC-rich. Of five Sp1 elements identified two had regulatory activity utilizing immortalized human keratinocytes: one element is located above the ostensible transcription initiation site, whereas the other is located within the 5'-untranslated region of the SULT2B1b mRNA. Sp1 and Sp2 transcription factors identified by supershift analyses induced reporter gene activity, an effect markedly augmented by histone deacetylase inhibition.
MeSH terms
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5' Untranslated Regions / genetics*
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Amino Acid Sequence
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Cell Line, Transformed
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DNA-Binding Proteins / metabolism*
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Genes, Reporter / genetics
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Histone Deacetylase Inhibitors
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Humans
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Isoenzymes / genetics
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Keratinocytes / enzymology
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Luciferases / analysis
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Luciferases / genetics
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Molecular Sequence Data
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Promoter Regions, Genetic*
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RNA, Messenger / genetics
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Response Elements*
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Sp1 Transcription Factor / metabolism*
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Sp2 Transcription Factor
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Sulfotransferases / genetics*
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Transcription Factors / metabolism*
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Transcription Initiation Site
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Transcriptional Activation*
Substances
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5' Untranslated Regions
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DNA-Binding Proteins
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Histone Deacetylase Inhibitors
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Isoenzymes
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RNA, Messenger
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SP2 protein, human
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Sp1 Transcription Factor
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Transcription Factors
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Sp2 Transcription Factor
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Luciferases
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Sulfotransferases
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SULT2B1 protein, human