Sp1 elements in SULT2B1b promoter and 5'-untranslated region of mRNA: Sp1/Sp2 induction and augmentation by histone deacetylase inhibition

FEBS Lett. 2005 Jul 4;579(17):3639-45. doi: 10.1016/j.febslet.2005.05.041.

Abstract

The steroid/sterol sulfotransferase gene (SULT2B1) encodes for two isozymes of which one (SULT2B1b) sulfonates cholesterol and is selectively expressed in skin. The human SULT2B1 gene contains neither a TATAAA nor a CCAAT motif upstream of the coding region for SULT2B1b; however, this area is GC-rich. Of five Sp1 elements identified two had regulatory activity utilizing immortalized human keratinocytes: one element is located above the ostensible transcription initiation site, whereas the other is located within the 5'-untranslated region of the SULT2B1b mRNA. Sp1 and Sp2 transcription factors identified by supershift analyses induced reporter gene activity, an effect markedly augmented by histone deacetylase inhibition.

MeSH terms

  • 5' Untranslated Regions / genetics*
  • Amino Acid Sequence
  • Cell Line, Transformed
  • DNA-Binding Proteins / metabolism*
  • Genes, Reporter / genetics
  • Histone Deacetylase Inhibitors
  • Humans
  • Isoenzymes / genetics
  • Keratinocytes / enzymology
  • Luciferases / analysis
  • Luciferases / genetics
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • RNA, Messenger / genetics
  • Response Elements*
  • Sp1 Transcription Factor / metabolism*
  • Sp2 Transcription Factor
  • Sulfotransferases / genetics*
  • Transcription Factors / metabolism*
  • Transcription Initiation Site
  • Transcriptional Activation*

Substances

  • 5' Untranslated Regions
  • DNA-Binding Proteins
  • Histone Deacetylase Inhibitors
  • Isoenzymes
  • RNA, Messenger
  • SP2 protein, human
  • Sp1 Transcription Factor
  • Transcription Factors
  • Sp2 Transcription Factor
  • Luciferases
  • Sulfotransferases
  • SULT2B1 protein, human