The non-receptor-associated tyrosine kinase Syk is a regulator of metastatic behavior in human melanoma cells

J Invest Dermatol. 2005 Jun;124(6):1293-9. doi: 10.1111/j.0022-202X.2005.23685.x.


Melanoma is one of the most aggressive neoplastic transformations and characterized by a high metastatic potential. The current study was performed to assess the impact of "spleen tyrosine kinase" (Syk), a non-receptor-associated tyrosine kinase, on growth and metastatic behavior of melanoma cells in vitro and in a severe combined immunodeficient (SCID)-mouse/human-melanoma xenotransplantation model in vivo. Syk was expressed in melanocytes but was found to be downregulated in melanoma cells. Vector-driven expression of Syk in two different melanoma cell lines did not influence growth speed, but significantly reduced the invasive growth potential of both cell lines in a Matrigel assay in vitro. In a SCID-mouse/human melanoma xenotransplantation model, Syk expressing Mel-Juso cells exhibited delayed and reduced tumor growth. After intravenous as well as subcutaneous injection of tumor cells, Syk-transfected cells formed significantly fewer metastatic tumor lesions than control cells. The presented data define Syk as a novel regulator of metastatic behavior of melanoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Division
  • Cell Line, Tumor
  • Enzyme Precursors / metabolism*
  • Female
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Melanoma / pathology
  • Melanoma / physiopathology*
  • Mice
  • Mice, SCID
  • Neoplasm Metastasis*
  • Neoplasm Transplantation
  • Protein-Tyrosine Kinases / metabolism*
  • Syk Kinase
  • Transplantation, Heterologous


  • Enzyme Precursors
  • Intracellular Signaling Peptides and Proteins
  • Protein-Tyrosine Kinases
  • SYK protein, human
  • Syk Kinase
  • Syk protein, mouse