Characterization of the mouse and rat relaxin receptors

Ann N Y Acad Sci. 2005 May;1041:8-12. doi: 10.1196/annals.1282.002.

Abstract

Rodent models have been used for many years to probe the actions of relaxin. Identification of the orthologs of human leucine-rich repeat-containing g-protein-coupled receptor 7 (LGR7), the relaxin receptor, in mouse and rat will enable characterization of the response of LGR7 to relaxin in these species. Partial LGR7 homologous sequences from mouse and rat were discovered in the Celera and NCBI gene databases, amplified, cloned, and sequenced. At the protein level, mouse and rat LGR7 are 85.2% and 85.7% identical to human LGR7. Mouse and rat LGR7 were able to bind to and be activated by relaxin ligands.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cloning, Molecular
  • Female
  • Humans
  • Mice
  • Pregnancy
  • RNA, Messenger / genetics
  • Rats
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism*
  • Receptors, Peptide / genetics
  • Receptors, Peptide / metabolism*
  • Relaxin / metabolism

Substances

  • RNA, Messenger
  • RXFP1 protein, mouse
  • Receptors, G-Protein-Coupled
  • Receptors, Peptide
  • Rxfp1 protein, rat
  • relaxin receptors
  • Relaxin