LGR7-truncate is a splice variant of the relaxin receptor LGR7 and is a relaxin antagonist in vitro

Ann N Y Acad Sci. 2005 May;1041:22-6. doi: 10.1196/annals.1282.005.

Abstract

The relaxin receptor (LGR7) and the insulin-like peptide 3 (INSL3) receptor (LGR8) are unique LGR family members in possessing a single, functionally important amino terminal LDL-A module.1 Mouse and rat cDNA was screened for LGR7 and LGR7 splice variant expression. A uterus-specific exon 4 deleted variant was identified and named LGR7-Truncate. Deletion of exon 4 results in a premature stop codon and a transcript that putatively encodes a secreted protein containing LGR7's LDL-A module. Expression of LGR7-Truncate with LGR7 in HEK-293T cells resulted in decreased relaxin-induced signaling of LGR7. LGR7-Truncate is potentially an endogenous regulator of LGR7 signaling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing / genetics*
  • Animals
  • Cell Line
  • Cyclic AMP / metabolism
  • Female
  • Humans
  • Mice
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Receptors, G-Protein-Coupled / genetics*
  • Receptors, G-Protein-Coupled / metabolism*
  • Receptors, Peptide / genetics*
  • Receptors, Peptide / metabolism*
  • Relaxin / antagonists & inhibitors*
  • Relaxin / metabolism
  • Uterus / metabolism

Substances

  • RNA, Messenger
  • RXFP1 protein, mouse
  • Receptors, G-Protein-Coupled
  • Receptors, Peptide
  • Rxfp1 protein, rat
  • Relaxin
  • Cyclic AMP