Studies on soluble ectodomain proteins of relaxin (LGR7) and insulin 3 (LGR8) receptors

Ann N Y Acad Sci. 2005 May;1041:35-9. doi: 10.1196/annals.1282.007.

Abstract

The ectodomains of both the relaxin (LGR7) and the INSL3 (LGR8) receptors can be expressed on the cell surface using only a single transmembrane domain. These membrane-anchored proteins retain the ability to bind relaxin and can be cleaved from the cell surface. The subsequent LGR7 protein, 7BP, binds relaxin and can act as a functional relaxin antagonist. By contrast, the equivalent LGR8 protein 8BP does not bind relaxin or antagonize LGR8 activity. The 7BP protein has been successfully immobilized onto chemically derivatized surfaces for the capture of relaxin peptides and subsequent identification via SELDI-MS analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Humans
  • Insulin / metabolism*
  • Mass Spectrometry
  • Membrane Proteins / chemistry*
  • Membrane Proteins / metabolism*
  • Protein Structure, Tertiary
  • Proteins / metabolism*
  • Receptors, G-Protein-Coupled / chemistry*
  • Receptors, G-Protein-Coupled / metabolism*
  • Receptors, Peptide
  • Relaxin / metabolism*
  • Solubility

Substances

  • Insulin
  • Leydig insulin-like protein
  • Membrane Proteins
  • Proteins
  • RXFP1 protein, human
  • RXFP2 protein, human
  • Receptors, G-Protein-Coupled
  • Receptors, Peptide
  • Relaxin