Molecular analysis of the En/Spm transposable element system of Zea mays

EMBO J. 1986 May;5(5):835-41.

Abstract

The nucleotide sequence of the autonomous transposable element En-1 isolated from the wx-844::En-1 allele has been determined. En-1 is 8287 bp long. The structure of the mosaic gene 1, coding for the major En transcript, has been established. The promoter gene 1 is located in the highly structured left end of the element and the gene spans almost the entire length of En-1. The first intron of gene 1 is 4434 nucleotides long and contains two large open reading frames, 2714 bp and 761 bp in size, which hybridize to minor RNA species in Northern blot experiments.

MeSH terms

  • Alleles
  • Base Sequence
  • Blotting, Northern
  • DNA Transposable Elements / genetics*
  • Genes, Plant / genetics
  • Introns / genetics
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • RNA, Plant / genetics
  • Zea mays / genetics*

Substances

  • DNA Transposable Elements
  • RNA, Plant