Enhanced clearance of Candida albicans from the oral cavities of mice following oral administration of Lactobacillus acidophilus

Clin Exp Immunol. 2005 Jul;141(1):29-36. doi: 10.1111/j.1365-2249.2005.02811.x.

Abstract

Orally administered live Lactobacillus acidophilus was assessed for its capacity to enhance clearance from the oral cavity of DBA/2 mice shown previously to be 'infection prone'. L. acidophilus fed to DBA/2 mice significantly shortened the duration of colonization of the oral cavity compared to controls. Enhanced clearance of Candida albicans correlated with both early mRNA gene expression for interleukin (IL)-4 and interferon (IFN)-gamma and expression of their secreted products in cultures of cervical lymph nodes stimulated with Candida antigen. In addition rapid clearance correlated with higher levels of IFN-gamma and nitric oxide in saliva. Delayed clearance, less pronounced levels of the cytokine response, saliva IFN-gamma and nitric oxide, and later mRNA expression for IL-4 and IFN-gamma relative to feeding with the L. acidophilus isolate were noted in mice fed a different Lactobacillus isolate (L. fermentum). These observations indicate significant variations in individual isolates to activate the common mucosal system.

MeSH terms

  • Administration, Oral
  • Animals
  • Candida albicans / isolation & purification*
  • Candidiasis, Oral / immunology
  • Candidiasis, Oral / microbiology
  • Candidiasis, Oral / therapy*
  • Cytokines / biosynthesis
  • Cytokines / genetics
  • Gene Expression
  • Immunity, Mucosal
  • Lactobacillus acidophilus*
  • Lymph Nodes / immunology
  • Male
  • Mice
  • Mice, Inbred DBA
  • Mouth / microbiology*
  • Neck
  • Nitric Oxide / biosynthesis
  • Probiotics / therapeutic use*
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Saliva / immunology

Substances

  • Cytokines
  • RNA, Messenger
  • Nitric Oxide