[18F]FDG uptake and PCNA, Glut-1, and Hexokinase-II expressions in cancers and inflammatory lesions of the lung

Neoplasia. 2005 Apr;7(4):369-79. doi: 10.1593/neo.04577.


Purpose: The aim of this study was to evaluate the relationships among [18F]fluorodeoxyglucose ([18F]-FDG) uptake, Glut-1 and HK-II expressions, and grade of inflammation in resected lung lesions.

Materials and methods: Sixty patients had undergone preoperative 18F-FDG-PET imaging and thoracotomy. For semiquantitative analysis of 18F-FDG uptake, partial volume effect corrected maximum standardized uptake values (pSUVs) were calculated. Immunohistochemical staining was performed in resected specimens using anti-Glut-1, anti-HK-II, and anti-proliferative cellular nuclear antigen (PCNA) antibodies, and immunoreactivities were scored as G-, H-, and P-indexes on a five-point scale (0: 0%; 1: 20%, 2: 40%; 3: 60%; 4: 80%, and 5: 100% percentages of strongly immunoreactive cells).Grade of inflammation was also evaluated.

Results: The malignant lesions had higher pSUV and higher G- and H- than nonmalignant lesions. pSUVs correlated with the G- (p < .001), H- (p < .01), and P-indexes (p < .01) in malignant lesions. In adenocarcinomas, cancers with lower differentiation showed higher expression of Glut-1 and HK-II than those with higher differentiation. A positive linear regression was observed between pSUVs and the grading of inflammation in nonmalignant lesions (p < .05).

Conclusions: Our study indicates that 18F-FDG uptake in lung cancer correlates well with the Glut-1, HK-II, and PCNA expression. For nonmalignant lesions, the presence of a higher inflammatory process correlated with 18F-FDG uptake.

MeSH terms

  • Adenocarcinoma / metabolism
  • Adult
  • Aged
  • Cell Differentiation
  • Female
  • Fluorodeoxyglucose F18 / pharmacokinetics*
  • Glucose Transporter Type 1
  • Hexokinase / metabolism*
  • Humans
  • Image Processing, Computer-Assisted
  • Immunohistochemistry
  • Inflammation
  • Linear Models
  • Lung / metabolism
  • Lung Neoplasms / diagnostic imaging*
  • Lung Neoplasms / metabolism
  • Male
  • Middle Aged
  • Monosaccharide Transport Proteins / metabolism*
  • Necrosis
  • Neoplasm Metastasis
  • Positron-Emission Tomography
  • Proliferating Cell Nuclear Antigen / metabolism*
  • Radiopharmaceuticals / pharmacokinetics*
  • Time Factors
  • Tuberculosis / metabolism


  • Glucose Transporter Type 1
  • Monosaccharide Transport Proteins
  • Proliferating Cell Nuclear Antigen
  • Radiopharmaceuticals
  • SLC2A1 protein, human
  • Fluorodeoxyglucose F18
  • Hexokinase