Characterisation of the immunoglobulin variable region gene usage encoding the murine anti-ganglioside antibody repertoire

J Neuroimmunol. 2005 Aug;165(1-2):92-103. doi: 10.1016/j.jneuroim.2005.04.011.


Neuropathogenic murine antibodies reactive with terminal disialylgalactose epitopes are innate and preferentially encoded by the VH7183.3b gene. Here we have studied antibodies reactive with internal galactose-linked disialosyl epitopes and the terminal trisaccharide of GT1b. Antibodies were of moderate affinity and unmutated. Anti-GD1b antibodies were often encoded by the VH10.2b heavy and gj38c light chain genes. Anti-GT1b antibodies with broader glycan binding patterns were encoded by VHQ52 and VHJ558 family genes. These data indicate that the discrete specificities of ganglioside-binding antibodies are dictated by particular patterns of V gene usage residing within the innate B cell repertoire.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / metabolism
  • Antibody Specificity
  • Binding Sites, Antibody
  • G(M1) Ganglioside / analogs & derivatives
  • G(M1) Ganglioside / immunology
  • G(M1) Ganglioside / metabolism
  • Gangliosides / administration & dosage
  • Gangliosides / immunology*
  • Gangliosides / metabolism
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain*
  • Gene Rearrangement, B-Lymphocyte, Light Chain*
  • Immunoglobulin Variable Region / biosynthesis
  • Immunoglobulin Variable Region / genetics*
  • Immunoglobulin Variable Region / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Molecular Sequence Data
  • Sialyltransferases / deficiency
  • Sialyltransferases / genetics
  • Surface Plasmon Resonance


  • Antibodies, Monoclonal
  • Gangliosides
  • Immunoglobulin Variable Region
  • ganglioside GD1alpha
  • G(M1) Ganglioside
  • trisialoganglioside GT1
  • Sialyltransferases
  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase