Abstract
Neuropathogenic murine antibodies reactive with terminal disialylgalactose epitopes are innate and preferentially encoded by the VH7183.3b gene. Here we have studied antibodies reactive with internal galactose-linked disialosyl epitopes and the terminal trisaccharide of GT1b. Antibodies were of moderate affinity and unmutated. Anti-GD1b antibodies were often encoded by the VH10.2b heavy and gj38c light chain genes. Anti-GT1b antibodies with broader glycan binding patterns were encoded by VHQ52 and VHJ558 family genes. These data indicate that the discrete specificities of ganglioside-binding antibodies are dictated by particular patterns of V gene usage residing within the innate B cell repertoire.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / biosynthesis*
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Antibodies, Monoclonal / metabolism
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Antibody Specificity
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Binding Sites, Antibody
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G(M1) Ganglioside / analogs & derivatives
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G(M1) Ganglioside / immunology
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G(M1) Ganglioside / metabolism
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Gangliosides / administration & dosage
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Gangliosides / immunology*
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Gangliosides / metabolism
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Gene Rearrangement, B-Lymphocyte, Heavy Chain*
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Gene Rearrangement, B-Lymphocyte, Light Chain*
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Immunoglobulin Variable Region / biosynthesis
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Immunoglobulin Variable Region / genetics*
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Immunoglobulin Variable Region / metabolism
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Molecular Sequence Data
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Sialyltransferases / deficiency
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Sialyltransferases / genetics
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Surface Plasmon Resonance
Substances
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Antibodies, Monoclonal
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Gangliosides
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Immunoglobulin Variable Region
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ganglioside GD1alpha
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G(M1) Ganglioside
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trisialoganglioside GT1
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Sialyltransferases
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alpha-N-acetylneuraminate alpha-2,8-sialyltransferase