Decreased interleukin-2 beta-chain receptor expression by interleukin-4 on LGL: influence on the IL-2 induced cytotoxicity and proliferation

Cancer Lett. 1992 May 30;64(1):43-9. doi: 10.1016/0304-3835(92)90020-v.

Abstract

Addition of increasing amounts of interleukin-4 (IL-4) to large granular lymphocytes (LGL) had a selective downregulative effect on the interleukin-2 beta-chain (p70) receptor expression. A 40% inhibition of the p70 expression compared to untreated cells was already observed after a 24-h incubation with IL-4. This decrease in p70 receptor expression had a marked suppressive effect on their proliferative response to IL-2. In addition, LGL cultured in the presence of both IL-2 and IL-4 substantially decreased the cytotoxic activity against the erythroleukemia cell line K562. Our data therefore indicate an important regulative role for IL-4 on the LAK-generation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division / drug effects
  • Cytotoxicity, Immunologic / drug effects
  • Gene Expression Regulation / drug effects
  • Humans
  • Interleukin-2 / metabolism*
  • Interleukin-2 / pharmacology
  • Interleukin-4 / pharmacology*
  • Lymphocytes / drug effects*
  • Lymphocytes / metabolism
  • Peptides / drug effects
  • Receptors, Interleukin-2 / drug effects*

Substances

  • Interleukin-2
  • Peptides
  • Receptors, Interleukin-2
  • Interleukin-4
  • poly(lysyl-(leucyl-poly-alanine))