A series of new antibacterial and antifungal Schiff's bases derived from sulfonamides, as well as their transition metal complexes incorporating cobalt (II), copper (II), nickel (II) and zinc (II) were synthesized, characterized and screened for their in-vitro antibacterial activity against six Gram-negative (Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella typhi and Shigella dysentriae) and four Gram-positive (Bacillus cereus, Corynebacterium diphtheriae, Staphylococcus aureous and Streptococcus pyogenes) bacterial strains and for in-vitro antifungal activity against Trichophyton longifusus, Candida albicans, Aspergillus flavus, Microsporum canis, Fusarium solani, Candida glaberata. The results of these studies show the metal complexes to be more antibacterial and antifungal as compared to the uncomplexed Schiffs' bases. The brine shrimp bioassay was also carried out to study the in-vitro cytotoxic properties of these synthesized ligands and their complexes.