Inhibition of cell proliferation and in vitro markers of angiogenesis by indole-3-carbinol, a major indole metabolite present in cruciferous vegetables

J Agric Food Chem. 2005 Jun 29;53(13):5164-9. doi: 10.1021/jf050034w.

Abstract

A variety of studies have suggested a cancer protective role of cruciferous vegetables. In the present study, we investigated the effect of indole-3-carbinol (I3C), a major indole metabolite in cruciferous vegetables, on cell proliferation and in vitro markers of angiogenesis in phorbol myristate acetate (PMA)-stimulated endothelial EA hy926 cells. The results showed that I3C inhibited the growth of EA hy926 cells in a concentration-dependent manner. The capillary-like tube formation by PMA-activated endothelial cells was significantly suppressed by I3C, and such inhibition was associated with decreased vascular endothelial growth factor (VEGF) and increased interleukin-8 (IL-8) secretion, but not with the expression of VEGF receptor-2 protein. Additionally, gelatin zymography analysis indicated that I3C suppressed activities of matrix metalloproteinases-2 (MMP-2) and MMP-9 stimulated by PMA. These results suggest that the dietary I3C may be useful in the treatment of human cancers and angiogenic diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brassicaceae / chemistry*
  • Cell Division / drug effects*
  • Cell Line
  • Endothelial Cells / cytology
  • Endothelial Cells / drug effects
  • Endothelial Cells / physiology
  • Enzyme Inhibitors / pharmacology
  • Humans
  • Indoles / pharmacology*
  • Interleukin-8 / metabolism
  • Matrix Metalloproteinase Inhibitors
  • Neovascularization, Physiologic / drug effects*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Vascular Endothelial Growth Factor A / metabolism
  • Vegetables / chemistry*

Substances

  • Enzyme Inhibitors
  • Indoles
  • Interleukin-8
  • Matrix Metalloproteinase Inhibitors
  • Vascular Endothelial Growth Factor A
  • indole-3-carbinol
  • Tetradecanoylphorbol Acetate