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. 2005 Jul;73(7):4395-8.
doi: 10.1128/IAI.73.7.4395-4398.2005.

Role of motility and flagellin glycosylation in the pathogenesis of Pseudomonas aeruginosa burn wound infections

Shiwani K Arora  1 , Alice N Neely, Barbara Blair, Stephen Lory, Reuben Ramphal
Affiliations

Role of motility and flagellin glycosylation in the pathogenesis of Pseudomonas aeruginosa burn wound infections

Shiwani K Arora et al. Infect Immun. 2005 Jul.

Abstract

In this study, we tested the contribution of flagellar motility, flagellin structure, and its glycosylation in Pseudomonas aeruginosa using genetically defined flagellar mutants. All mutants and their parent strains were tested in a burned-mouse model of infection. Motility and glycosylation of the flagellum appear to be important determinants of flagellar-mediated virulence in this model. This is the first report where genetically defined flagellar variants of P. aeruginosa were tested in the burned-mouse model of infection.

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Figures

FIG. 1.
FIG. 1.
Motility phenotype of different P. aeruginosa strains used in this study. Panel A shows the motile wild-type P. aeruginosa strains PAK and PAO1 and mutant strains that were defective in motility because of mutations in the fliC gene (PAKΔC and PAOC−) or motABCD genes (PAKmotAB). Panel B shows the glycosylation mutant strains PAKrfbC and PAOrfbC, which were both motile. Panel C shows the complemented strains in which fliC mutant strain PAKΔC was chromosomally complemented with different flagellins with their promoters.
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