Parameters for measurement of oxidative stress in diabetes mellitus: applicability of enzyme-linked immunosorbent assay for clinical evaluation

J Investig Med. 2005 May;53(4):167-75. doi: 10.2310/6650.2005.00403.


Investigations of the mechanisms involved in the onset and progression of diabetes have recently confronted the role of reactive oxygen species (ROS) and oxidative stress. Prolonged exposure to hyperglycemic conditions induces nonenzymatic glycation of protein via the so-called Maillard reaction, resulting in Schiff-base products and Amadori products that engender ROS production. These processes initiate and exacerbate micro- and macrovascular complications in diabetes. Increased oxidative stress is induced by excessive ROS production and inadequate antioxidant defenses. Recently, oxidative stress status markers have been associated directly with the severity and prognosis of diabetes. To examine oxidative stress, reliable and high-throughput methods are needed to examine large numbers of clinical samples. The emerging availability of enzyme-linked immunosorbent assay (ELISA) for oxidative stress status markers allows its application to assessment of various pathophysiologic conditions, including diabetes. This review outlines the recent achievements of ELISA application for clinical studies elucidating oxidative stress. It introduces the potential applicability of ELISA for investigating oxidative stress in diabetes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Bilirubin / metabolism
  • Carrier Proteins / metabolism
  • DNA / metabolism
  • Diabetes Mellitus / metabolism*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Fatty Acid-Binding Proteins
  • Glycation End Products, Advanced
  • Humans
  • Lipid Metabolism
  • Nitric Oxide / biosynthesis
  • Oxidation-Reduction
  • Oxidative Stress*


  • Carrier Proteins
  • Fatty Acid-Binding Proteins
  • Glycation End Products, Advanced
  • Nitric Oxide
  • DNA
  • Bilirubin