Oligofructose promotes satiety in rats fed a high-fat diet: involvement of glucagon-like Peptide-1

Obes Res. 2005 Jun;13(6):1000-7. doi: 10.1038/oby.2005.117.


Objective: To analyze the putative interest of oligofructose (OFS) in the modulation of food intake after high-fat diet in rats and to question the relevance of the expression and secretion of intestinal peptides in that context.

Research methods and procedures: Male Wistar rats were pretreated with standard diet or OFS-enriched (10%) standard diet for 35 days followed by 15 days of high-fat diet enriched or not with OFS (10%) treatment. Body weight, food intake, triglycerides, and plasma ghrelin levels were monitored during the treatment. On day 50, rats were food-deprived 8 hours and anesthetized for blood and intestinal tissue sampling for further proglucagon mRNA, glucagon-like peptide (GLP)-1, and GLP-2 quantification.

Results: The addition of OFS in the diet protects against the promotion of energy intake, body weight gain, fat mass development, and serum triglyceride accumulation induced by a high-fat diet. OFS fermentation leads to an increase in proglucagon mRNA in the cecum and the colon and in GLP-1 and GLP-2 contents in the proximal colon, with consequences on the portal concentration of GLP-1 (increase). A lower ghrelin level is observed only when OFS is added to the standard diet of rats.

Discussion: In rats exposed to high-fat diet, OFS is, thus, able to modulate endogenous production of gut peptides involved in appetite and body weight regulation. Because several approaches are currently used to treat type 2 diabetes and obesity with limited effectiveness, dietary fibers such as OFS, which promote the endogenous production of gut peptides like GLP-1, could be proposed as interesting nutrients to consider in the management of fat intake and associated metabolic disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Weight / physiology
  • Dietary Fats / administration & dosage*
  • Dietary Fats / metabolism
  • Dipeptidyl Peptidase 4 / metabolism
  • Eating / drug effects
  • Eating / physiology
  • Ghrelin
  • Glucagon / blood
  • Glucagon / genetics
  • Glucagon / metabolism*
  • Glucagon-Like Peptide 1
  • Glucagon-Like Peptide 2
  • Intestinal Mucosa / metabolism
  • Liver / metabolism
  • Male
  • Obesity / blood
  • Obesity / drug therapy*
  • Obesity / metabolism
  • Oligosaccharides / metabolism
  • Oligosaccharides / pharmacology*
  • Peptide Fragments / blood
  • Peptide Fragments / genetics
  • Peptide Fragments / metabolism*
  • Peptide Hormones / blood
  • Peptide Hormones / metabolism
  • Peptides / blood
  • Peptides / metabolism
  • Proglucagon
  • Protein Precursors / blood
  • Protein Precursors / genetics
  • Protein Precursors / metabolism*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction
  • Triglycerides / blood


  • Dietary Fats
  • Ghrelin
  • Glucagon-Like Peptide 2
  • Oligosaccharides
  • Peptide Fragments
  • Peptide Hormones
  • Peptides
  • Protein Precursors
  • RNA, Messenger
  • Triglycerides
  • oligofructose
  • Proglucagon
  • Glucagon-Like Peptide 1
  • Glucagon
  • Dipeptidyl Peptidase 4