[Alogenic islet transplantation on the rat liver after alogenic dendritic cells injection in the thymus]

Arq Gastroenterol. 2005 Jan-Mar;42(1):41-9. doi: 10.1590/s0004-28032005000100010. Epub 2005 Jun 22.
[Article in Portuguese]


Background: The major indication for pancreas or islet transplantation is diabetes mellitus type I. This process has to supply the insulin necessity keeping glucose under control.

Aim: We studied alogenic islet transplantation on the rat liver, Wistar (RT1u) to Lewis (RT1(1)) as a recipient. Control group (n = 8) and dendritic cell group (n = 9) respectively with injection of Hanks solution and dendritic cells in the thymus before islet transplantation.

Material and methods: With the method of isolation and purification of the islets we obtained both in the control group 3637 +/- 783,3 islets with purity of 85 +/- 3,52% and dendritic cell group 3268 +/- 378 islets with purity of 87 +/- 4,47%. The dendritic cells were retrieved from the spleen and we obtained 3,34 x 105+/-1,16 cells. Diabetes was induced by i.v. streptozotocin.

Results: Control group the transplantation of 3637 +/- 783,3 islets in the rat liver normalized glucose test, 7,21 +/- 0,57 mmol/L in the second post-operative day. Acute rejection came in the 10 postoperative day with significantly increase of glucose test. Dendritic cell group, the transplantation of 3258 +/- 378 islets in the rat liver, normalized the glucose test was 9,3 +/- 2,85 mmoL/L in the second postoperative day. From the 4th postoperative day to 10th postoperative day the glucose test increase significantly showing an early acute rejection.

Conclusion: The injection of dendritic cells in the thymus before alogenic islet transplantation in the rat liver lead to an early acute rejection.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Cell Separation / methods
  • Dendritic Cells / cytology*
  • Dendritic Cells / immunology
  • Diabetes Mellitus, Experimental / surgery*
  • Female
  • Graft Survival
  • Islets of Langerhans Transplantation / immunology
  • Islets of Langerhans Transplantation / methods*
  • Male
  • Pancreatectomy
  • Rats
  • Rats, Inbred Lew
  • Rats, Wistar
  • Streptozocin
  • Thymus Gland*
  • Transplantation, Isogeneic


  • Streptozocin