Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana

FEBS J. 2005 Jul;272(13):3461-76. doi: 10.1111/j.1742-4658.2005.04770.x.

Abstract

Eukaryotic cells respond to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). In this case, so-called unfolded protein response (UPR) genes are induced. We determined the transcriptional expression of Arabidopsis thaliana UPR genes by fluid microarray analysis of tunicamycin-treated plantlets. Two hundred and fifteen up-regulated genes and 17 down-regulated ones were identified. These genes were reanalyzed with functional DNA microarrays, using DNA fragments cloned through fluid microarray analysis. Finally, 36 up-regulated and two down-regulated genes were recognized as UPR genes. Among them, the up-regulation of genes related to protein degradation (HRD1, SEL-1L/HRD3 and DER1), regulation of translation (P58(IPK)), and apoptosis (BAX inhibitor-1) was reconfirmed by real-time reverse transcriptase-PCR. The induction of SEL-1L protein in an Arabidopsis membrane fraction on tunicamycin-treatment was demonstrated. Phosphorylation of initiation factor-2alpha, which was inhibited by P58(IPK), was decreased in tunicamycin-treated plantlets. However, regulatory changes in translation caused by ER stress were not detected in Arabidopsis. Plant cells appeared to have a strategy for overcoming ER stress through enhancement of protein folding activity, degradation of unfolded proteins, and regulation of apoptosis, but not regulation of translation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Endoplasmic Reticulum / metabolism*
  • Eukaryotic Initiation Factor-2 / genetics
  • Eukaryotic Initiation Factor-2 / metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant*
  • Gene Library
  • Molecular Sequence Data
  • Phosphorylation / drug effects
  • Plant Proteins
  • Plasmids
  • Protein Folding*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tunicamycin / pharmacology
  • eIF-2 Kinase / genetics
  • eIF-2 Kinase / metabolism

Substances

  • Anti-Bacterial Agents
  • Arabidopsis Proteins
  • Eukaryotic Initiation Factor-2
  • P58IPK protein, plant
  • Plant Proteins
  • RNA, Messenger
  • RNA, Plant
  • Recombinant Proteins
  • Repressor Proteins
  • Tunicamycin
  • eIF-2 Kinase

Associated data

  • GENBANK/AB197676