PA 20, a semi-selective medium for isolation and enumeration of Pantoea ananatis

Teresa Goszczynska; Stephanus N Venter; Teresa A Coutinho

doi:10.1016/j.mimet.2005.05.004

PA 20, a semi-selective medium for isolation and enumeration of Pantoea ananatis

J Microbiol Methods. 2006 Feb;64(2):225-31. doi: 10.1016/j.mimet.2005.05.004. Epub 2005 Jun 23.

Authors

Teresa Goszczynska¹, Stephanus N Venter, Teresa A Coutinho

Affiliation

¹ Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa. goszczynskat@arc.agric.za

PMID: 15979177
DOI: 10.1016/j.mimet.2005.05.004

Abstract

A semi-selective medium, PA 20, was developed for the isolation and enumeration of Pantoea ananatis from plant material, specifically from onion seed. The medium has a pH of 8.0 and contains NH4H2PO4, K2HPO4, magnesium sulphate, NaCl, d (+) arabitol, crystal violet, bromothymol blue and thallium nitrate. All P. ananatis strains from a variety of hosts produced characteristic yellow colonies in 6-7 days at 25 degrees C. Plating efficiencies on PA 20 in comparison to nutrient agar ranged from 92 to 112%. Recovery from naturally infested and artificially contaminated onion seed was high, with an almost total reduction of saprophytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Culture Media*
Onions / microbiology
Pantoea / growth & development
Pantoea / isolation & purification*
Seeds / microbiology
Sensitivity and Specificity

Substances

Culture Media