Low rate of occult hepatitis B virus infection among anti-HBc positive blood donors living in a low prevalence region in Brazil

J Infect. 2005 Jul;51(1):24-9. doi: 10.1016/j.jinf.2004.07.007. Epub 2004 Sep 30.


Objective: The aim of this study was to determine the rate of occult hepatitis B virus (HBV) infection among blood donors living in a geographic region of low (5.6%) anti-HBc prevalence.

Subjects and methods: Sera from 150 candidate blood donors whose blood was rejected due to total anti-HBc reactivity (despite absence of HBsAg) were tested for anti-HBs and IgM anti-HBc antibodies, as well as for HBeAg/anti-HBe. Serum HBV DNA was sought by using a PCR assay able to amplify part of the surface gene. Viral load was measured in the PCR positive samples.

Results: The pattern 'anti-HBc alone' (without HBsAg and anti-HBs antibodies) was found in 64 (42.7%) subjects. IgM anti-HBc and anti-HBe antibodies were detected in 2 (1.3%) and 80 (53.3%) samples, respectively. No sample was HBeAg-reactive. HBV DNA was repeatedly found in five (3.3%) samples, three of which were anti-HBs positive and two anti-HBs negative. All five HBV DNA positive samples showed a low viral load (<1000copies/ml).

Conclusions: The data indicated a low rate of occult infection among anti-HBc positive, HBsAg negative blood donors living in a region of low prevalence of infection. Viral load was very low in all HBV infected subjects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Blood Donors*
  • Brazil / epidemiology
  • DNA, Viral / blood
  • Female
  • Hepatitis B / epidemiology*
  • Hepatitis B Antibodies / blood*
  • Hepatitis B Core Antigens / immunology
  • Humans
  • Male
  • Middle Aged
  • Prevalence
  • Viral Load


  • DNA, Viral
  • Hepatitis B Antibodies
  • Hepatitis B Core Antigens