Functional assessment of vascular reactivity after chronic intermittent hypoxia in the rat

Respir Physiol Neurobiol. 2006 Feb 28;150(2-3):278-86. doi: 10.1016/j.resp.2005.05.020. Epub 2005 Jun 24.


We recently developed a model of chronic intermittent hypoxia (CIH) (FiO2 5%, 8 h/day, 35 days) in the rat that was associated with an increased infarction in isolated heart. The aim of the present study was to characterize its functional consequences on vasoreactivity. Aorta and carotid artery were studied using organ bath technique while mesenteric vascular bed was perfused. In the three vascular beds, relaxation to acetylcholine was similar in CIH and control normoxic (NX) rats. Contractions to noradrenaline and angiotensin II were similar between CIH and NX rats. In contrast, contraction to endothelin-1 was increased by 17% (P < 0.05) in carotid artery from CIH rats. Indomethacin pre-treatment reduced by 24% (P < 0.001) contraction to endothelin-1 in carotid artery from CIH rats only. These data suggested that 35-day CIH-exposure induced no change in endothelial function of aorta, carotid artery and mesenteric bed. In contrast, CIH-exposure induced an increased contractile response to endothelin-1 in carotid artery, presumably owing to the release of constrictor cyclooxygenase-derived products.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Aorta / drug effects
  • Aorta / physiopathology*
  • Carotid Arteries / drug effects
  • Carotid Arteries / physiopathology*
  • Chronic Disease
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Endothelin-1 / pharmacology
  • Hypoxia, Brain / physiopathology*
  • In Vitro Techniques
  • Male
  • Mesenteric Arteries / drug effects
  • Mesenteric Arteries / physiopathology
  • Potassium Chloride / pharmacology
  • Rats
  • Rats, Wistar
  • Vasoconstriction / drug effects
  • Vasoconstriction / physiology*
  • Vasoconstrictor Agents / pharmacology


  • Endothelin-1
  • Vasoconstrictor Agents
  • Potassium Chloride