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. 2005 Jul 1;33(Web Server issue):W311-4.
doi: 10.1093/nar/gki404.

MutDB Services: Interactive Structural Analysis of Mutation Data

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Free PMC article

MutDB Services: Interactive Structural Analysis of Mutation Data

Jessica Dantzer et al. Nucleic Acids Res. .
Free PMC article

Abstract

Non-synonymous single nucleotide polymorphisms (SNPs) and mutations have been associated with human phenotypes and disease. As more and more SNPs are mapped to phenotypes, understanding how these variations affect the function and expression of genes and gene products becomes an important endeavor. We have developed a set of tools to aid in the understanding of how amino acid substitutions affect protein structures. To do this, we have annotated SNPs in dbSNP and amino acid substitutions in Swiss-Prot with protein structural information, if available. We then developed a novel web interface to this data that allows for visualization of the location of these substitutions. We have also developed a web service interface to the dataset and developed interactive plugins for UCSF's Chimera structural modeling tool and PyMOL that integrate our annotations with these sophisticated structural visualization and modeling tools. The web services portal and plugins can be downloaded from http://www.lifescienceweb.org/ and the web interface is at http://www.mutdb.org/.

Figures

Figure 1
Figure 1
Visualization of mutations through web service-enabled client applications. (a) UCSF Chimera is shown with the mutation web service controller window showing a mutation in the TP53 gene. (b) PyMOL is shown with the mutation web service controller window showing a mutation in the BRCA1 gene.
Figure 2
Figure 2
Web interface for structural visualization of mutation data. A mutation in TP53 is highlighted showing an aspartate-to-glutamate substitution.

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