Antibody response to a non-conserved C-terminal part of human histone deacetylase 3 in colon cancer patients

Int J Cancer. 2005 Dec 10;117(5):800-6. doi: 10.1002/ijc.21240.


Antibodies to cancer antigens can often be detected in the sera of patients, although the mechanism of the underlying humoral immune response is poorly understood. Using immunoscreening of tumor-derived cDNA expression libraries (SEREX), we identified human histone deacetylase 3 (HDAC3) as serologically defined antigen in colon cancer. Closely related HDAC1 and HDAC2 do not elicit humoral response in colon cancer patients. We show that the C-terminal region of HDAC3 protein lacking the homology to other Class I HDAC contains at least 3 distinct B-cell epitopes that are recognized by the serum antibodies. HDAC3 in combination with other SEREX antigens may become a useful molecular biomarker with diagnostic or prognostic value for a subset of colon cancer patients.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Neoplasm / biosynthesis*
  • B-Lymphocytes / immunology
  • Base Sequence
  • Blotting, Western
  • Colonic Neoplasms / enzymology*
  • Colonic Neoplasms / immunology*
  • DNA Primers
  • Epitopes / immunology
  • Histone Deacetylases / chemistry*
  • Histone Deacetylases / immunology
  • Humans
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid


  • Antibodies, Neoplasm
  • DNA Primers
  • Epitopes
  • Histone Deacetylases
  • histone deacetylase 3