Abstract
The p50 subunit of the NF-kappa B transcription complex is derived from the N-terminal half of a larger precursor protein, p105. Although a fair amount is known about functions located within the p50 sequences, less is known about the C-terminal half of p105. In this report, we have identified a potent transcription activation domain located in the C terminus of mouse p105. In addition, the I kappa B beta proteins chicken p40 and human MAD-3, proteins that are related to the p105 C terminus, strongly activated transcription in chicken cells and yeast when fused to GAL4 DNA-binding sequences. Furthermore, chicken p40 is primarily located in the nucleus of chicken cells when overexpressed from a retroviral vector. Our results suggest novel models for the function and regulation of NF-kappa B transcription complexes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Chickens
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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Fluorescent Antibody Technique
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Fungal Proteins / genetics
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Gene Expression Regulation / genetics
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Humans
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I-kappa B Proteins*
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Mice
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Molecular Sequence Data
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NF-KappaB Inhibitor alpha
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NF-kappa B / antagonists & inhibitors*
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NF-kappa B / chemistry
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NF-kappa B / genetics*
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NF-kappa B p50 Subunit
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Plasmids / genetics
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Protein Precursors / chemistry
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Protein Precursors / genetics*
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Recombinant Fusion Proteins / genetics*
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Saccharomyces cerevisiae
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Saccharomyces cerevisiae Proteins*
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Transcription Factors / genetics
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Transcription, Genetic / genetics*
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Tumor Cells, Cultured
Substances
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DNA-Binding Proteins
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Fungal Proteins
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GAL4 protein, S cerevisiae
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I kappa B beta protein
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I-kappa B Proteins
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NF-kappa B
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NF-kappa B p50 Subunit
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NFKBIA protein, human
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Nfkbia protein, mouse
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Protein Precursors
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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NF-KappaB Inhibitor alpha