The C terminus of the NF-kappa B p50 precursor and an I kappa B isoform contain transcription activation domains

Nucleic Acids Res. 1992 May 25;20(10):2453-8. doi: 10.1093/nar/20.10.2453.


The p50 subunit of the NF-kappa B transcription complex is derived from the N-terminal half of a larger precursor protein, p105. Although a fair amount is known about functions located within the p50 sequences, less is known about the C-terminal half of p105. In this report, we have identified a potent transcription activation domain located in the C terminus of mouse p105. In addition, the I kappa B beta proteins chicken p40 and human MAD-3, proteins that are related to the p105 C terminus, strongly activated transcription in chicken cells and yeast when fused to GAL4 DNA-binding sequences. Furthermore, chicken p40 is primarily located in the nucleus of chicken cells when overexpressed from a retroviral vector. Our results suggest novel models for the function and regulation of NF-kappa B transcription complexes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chickens
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • Fluorescent Antibody Technique
  • Fungal Proteins / genetics
  • Gene Expression Regulation / genetics
  • Humans
  • I-kappa B Proteins*
  • Mice
  • Molecular Sequence Data
  • NF-KappaB Inhibitor alpha
  • NF-kappa B / antagonists & inhibitors*
  • NF-kappa B / chemistry
  • NF-kappa B / genetics*
  • NF-kappa B p50 Subunit
  • Plasmids / genetics
  • Protein Precursors / chemistry
  • Protein Precursors / genetics*
  • Recombinant Fusion Proteins / genetics*
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / genetics
  • Transcription, Genetic / genetics*
  • Tumor Cells, Cultured


  • DNA-Binding Proteins
  • Fungal Proteins
  • GAL4 protein, S cerevisiae
  • I kappa B beta protein
  • I-kappa B Proteins
  • NF-kappa B
  • NF-kappa B p50 Subunit
  • NFKBIA protein, human
  • Nfkbia protein, mouse
  • Protein Precursors
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • NF-KappaB Inhibitor alpha