Involvement of equine chorionic gonadotropin (eCG) carbohydrate side chains in its bioactivity; lessons from recombinant hormone expressed in insect cells

Reprod Nutr Dev. 2005 May-Jun;45(3):255-9. doi: 10.1051/rnd:2005018.

Abstract

Natural eCG consists of as much as 45% carbohydrate side chains. The present paper deals with the analysis of the roles of the N- and O-linked saccharides of this hormone in the different steps of its activity and its possible replacement by recombinant eCG expressed in baculovirus-insect cell systems.

Publication types

  • Review

MeSH terms

  • Animals
  • Baculoviridae
  • Biological Assay
  • Cell Line
  • Female
  • Glycoside Hydrolases / genetics
  • Glycosyltransferases / genetics
  • Gonadotropins, Equine / chemistry
  • Gonadotropins, Equine / metabolism
  • Gonadotropins, Equine / physiology*
  • Horses
  • Insecta
  • Male
  • Oligosaccharides / metabolism*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Spodoptera

Substances

  • Gonadotropins, Equine
  • Oligosaccharides
  • Recombinant Proteins
  • Glycosyltransferases
  • Glycoside Hydrolases