Production of interleukin 8 by cultured synovial cells in response to interleukin 1 and tumor necrosis factor

K Hirota; T Akahoshi; H Endo; H Kondo; S Kashiwazaki

doi:10.1007/BF00246871

Production of interleukin 8 by cultured synovial cells in response to interleukin 1 and tumor necrosis factor

Rheumatol Int. 1992;12(1):13-6. doi: 10.1007/BF00246871.

Authors

K Hirota¹, T Akahoshi, H Endo, H Kondo, S Kashiwazaki

Affiliation

¹ Department of Internal Medicine, Kitasato University School of Medicine, Kanagawa, Japan.

PMID: 1598496
DOI: 10.1007/BF00246871

Abstract

Both interleukin 1 alpha (IL-1 alpha) and tumor necrosis factor alpha (TNF alpha) stimulated the production of interleukin 8 (IL-8) by synovial cells in time and dose dependent manners. Enhanced chemotactic activity of polymorphonuclear cells (PMN) in culture supernatants of synovial cells was neutralized with anti-IL-8 antibody, thus showing synovial cells to be capable of secreting IL-8 which may contribute to PMN accumulation in rheumatoid inflamed joints.

MeSH terms

Cells, Cultured
Chemotaxis / drug effects
Chemotaxis / physiology
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Dose-Response Relationship, Drug
Humans
Interleukin-1 / pharmacology*
Interleukin-8 / analysis
Interleukin-8 / metabolism*
Neutrophils / drug effects
Neutrophils / physiology
Radioimmunoassay
Synovial Membrane / cytology*
Synovial Membrane / drug effects
Synovial Membrane / metabolism*
Time Factors
Tumor Necrosis Factor-alpha / pharmacology*

Substances

Interleukin-1
Interleukin-8
Tumor Necrosis Factor-alpha
Dactinomycin
Cycloheximide