ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis

Glycobiology. 2005 Nov;15(11):1156-63. doi: 10.1093/glycob/cwj002. Epub 2005 Jun 29.


N-linked protein glycosylation follows a conserved pathway in eukaryotic cells. The assembly of the lipid-linked core oligosaccharide Glc3Man9GlcNAc2, the substrate for the oligosaccharyltransferase (OST), is catalyzed by different glycosyltransferases located at the membrane of the endoplasmic reticulum (ER). The substrate specificity of the different glycosyltransferase guarantees the ordered assembly of the branched oligosaccharide and ensures that only completely assembled oligosaccharide is transferred to protein. The glycosyltransferases involved in this pathway are highly specific, catalyzing the addition of one single hexose unit to the lipid-linked oligosaccharide (LLO). Here, we show that the dolichylphosphomannose-dependent ALG9 mannosyltransferase is the exception from this rule and is required for the addition of two different alpha-1,2-linked mannose residues to the LLO. This report completes the list of lumen-oriented glycosyltransferases required for the assembly of the LLO.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dolichol Monophosphate Mannose / metabolism
  • Endoplasmic Reticulum / metabolism
  • Lipids / physiology*
  • Mannose / metabolism
  • Mannosyltransferases / metabolism*
  • Models, Biological
  • Oligosaccharides / biosynthesis*
  • Saccharomyces cerevisiae / enzymology


  • Lipids
  • Oligosaccharides
  • Dolichol Monophosphate Mannose
  • ALG9 protein, S cerevisiae
  • Mannosyltransferases
  • Mannose