Radioprotective property of the ethanolic extract of Piper betel Leaf

J Radiat Res. 2005 Jun;46(2):165-71. doi: 10.1269/jrr.46.165.


The radioprotective activity of Piper betel ethanolic extract (PE) has been studied using rat liver mitochondria and pBR 322 plasmid DNA as two model in vitro systems. The extract effectively prevented gamma-ray induced lipid peroxidation as assessed by measuring thiobarbituric acid reactive substrates, lipid hydroperoxide and conjugated diene. Likewise, it prevented radiation-induced DNA strand breaks in a concentration dependent manner. The radioprotective activity of PE could be attributed to its hydroxyl and superoxide radicals scavenging property along with its lymphoproliferative activity. The radical scavenging capacity of PE was primarily due to its constituent phenolics, which were isolated and identified as chevibetol and allyl pyrocatechol.

MeSH terms

  • Animals
  • Cells, Cultured
  • DNA Damage / drug effects*
  • Dose-Response Relationship, Drug
  • Dose-Response Relationship, Radiation
  • Ethanol / chemistry
  • Lipid Peroxidation / drug effects
  • Lipid Peroxidation / radiation effects
  • Male
  • Mitochondria, Liver / drug effects*
  • Mitochondria, Liver / radiation effects*
  • Piper betle / chemistry*
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry*
  • Plasmids / drug effects*
  • Plasmids / radiation effects*
  • Radiation Dosage
  • Radiation Tolerance / drug effects
  • Radiation-Protective Agents / chemistry
  • Radiation-Protective Agents / pharmacology
  • Rats
  • Rats, Wistar


  • Plant Extracts
  • Radiation-Protective Agents
  • Ethanol