Sulforaphane prevents mouse skin tumorigenesis during the stage of promotion

Cancer Lett. 2006 May 8;236(1):72-9. doi: 10.1016/j.canlet.2005.05.007. Epub 2005 Jul 1.

Abstract

Sulforaphane (SF), a natural product from broccoli, is known to enhance detoxification of carcinogens and block initiation of chemically-induced carcinogenesis in animal models. Cell culture and xenograft studies suggest additional roles for SF, inhibiting growth of tumors, arresting the cell cycle and enhancing apoptosis. As currently reported, topical SF (1, 5 or 10 micromol/mouse) significantly inhibited 7,12-dimethylbenz(a)anthracene/12-O-tetradecanoylphorbol 13-acetate (TPA)-induced mouse skin tumorigenesis, using either an anti-promotion protocol (SF from 1 week after carcinogen until the end of the study) or a combined anti-initiation, anti-promotion protocol (SF 7 days prior to carcinogen until the end of the study). Surprisingly, no significant effect was observed in an anti-initiation protocol (SF from 7 days prior to 7 days after carcinogen). Separately, SF inhibited TPA-induced ornithine decarboxylase activity in mouse skin, an obligate step in TPA-induced promotion of carcinogenesis. These data link this molecular mechanism to SF-dependent inhibition of the promotion of tumorigenesis.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • 9,10-Dimethyl-1,2-benzanthracene
  • Administration, Topical
  • Animals
  • Anticarcinogenic Agents / administration & dosage
  • Anticarcinogenic Agents / pharmacology*
  • Brassica
  • Carcinogens
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / administration & dosage
  • Enzyme Inhibitors / pharmacology
  • Female
  • Isothiocyanates
  • Mice
  • Ornithine Decarboxylase / metabolism
  • Ornithine Decarboxylase Inhibitors
  • Skin / drug effects
  • Skin / enzymology
  • Skin Neoplasms / chemically induced
  • Skin Neoplasms / prevention & control*
  • Tetradecanoylphorbol Acetate
  • Thiocyanates / administration & dosage
  • Thiocyanates / isolation & purification
  • Thiocyanates / pharmacology*
  • Time Factors

Substances

  • Anticarcinogenic Agents
  • Carcinogens
  • Enzyme Inhibitors
  • Isothiocyanates
  • Ornithine Decarboxylase Inhibitors
  • Thiocyanates
  • 9,10-Dimethyl-1,2-benzanthracene
  • Ornithine Decarboxylase
  • sulforaphane
  • Tetradecanoylphorbol Acetate