Aberrant EGFR and chromosome 7 associate with outcome in laryngeal cancer

Laryngoscope. 2005 Jul;115(7):1212-8. doi: 10.1097/01.MLG.0000163755.21035.8F.


Objectives/hypothesis: Epidermal growth factor receptor (EGFR) over-expression has been reported as a prognostic indicator in laryngeal cancer; however, the association with disease outcome has been inconsistent among studies. Here, we use fluorescence in situ hybridization (FISH) in addition to immunohistochemistry to assess laryngeal squamous cell carcinoma (SCC) to determine whether FISH can better predict patient outcome.

Study design: Retrospective study on 59 patients presenting with advanced disease.

Methods: EGFR and chromosome 7 genomic statuses were measured using FISH, and EGFR expression was assessed by immunohistochemistry on formalin-fixed, paraffin-embedded specimens and correlated with outcome in the 59 patients.

Results: EGFR expression was marginally associated with outcome, whereas both EGFR and chromosome 7 FISH status were significantly associated with outcome, and the combination of EGFR and chromosome 7 FISH status provided the strongest association of any two combined parameters (P = .0004). Combining EGFR expression with EGFR and chromosome 7 FISH status provided further improvement (P > .0001).

Conclusions: Measurements of EGFR and chromosome 7 FISH status, and to a lesser extent EGFR expression, have potential value in treatment planning for patients with laryngeal SCC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Chromosome Aberrations
  • Chromosomes, Human, Pair 7 / genetics*
  • Female
  • Genes, erbB-1 / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Laryngeal Neoplasms / genetics*
  • Laryngeal Neoplasms / mortality*
  • Laryngeal Neoplasms / pathology
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Prognosis
  • Retrospective Studies
  • Survival Rate