Membranous osteogenesis system modeled with KUSA-A1 mature osteoblasts

Biochim Biophys Acta. 2005 Aug 30;1725(1):57-63. doi: 10.1016/j.bbagen.2005.05.027.


Several stromal cells were established from murine bone marrow cultures. One of the KUSA subclones, KUSA-A1 cells, displays osteogenic characteristics in vitro and in vivo. The calcium deposition, osteocalcin release, and parathyroid hormone (PTH) responsiveness of KUSA-A1 cells indicate that they are mature osteoblasts or osteocytes. Bone had formed in subcutaneous tissue 1 week after subcutaneous injection of cells into immunodeficient mice. The osteogenesis by KUSA-A1 was not mediated by chondrogenesis and thus was considered to be membranous ossification. These unique characteristics of KUSA-A1 cells provide an opportunity to analyze the process of membranous ossification in detail.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcification, Physiologic
  • Cell Differentiation
  • Cell Line
  • Cell Shape
  • Cells, Cultured
  • Female
  • Gap Junctions / physiology
  • Mice
  • Microscopy, Electron
  • Models, Biological*
  • Osteoblasts / cytology*
  • Osteoblasts / physiology*
  • Osteoblasts / ultrastructure
  • Osteogenesis / physiology*
  • Stromal Cells / cytology
  • Stromal Cells / metabolism