Resolution of N-linked oligosaccharides in glycoproteins based upon transglycosylation reaction by CE-TOF-MS

Jun Zhe Min; Toshimasa Toyo'oka; Masaru Kato; Takeshi Fukushima

doi:10.1039/b505744b

Resolution of N-linked oligosaccharides in glycoproteins based upon transglycosylation reaction by CE-TOF-MS

Chem Commun (Camb). 2005 Jul 21:(27):3484-6. doi: 10.1039/b505744b. Epub 2005 Jun 9.

Authors

Jun Zhe Min¹, Toshimasa Toyo'oka, Masaru Kato, Takeshi Fukushima

Affiliation

¹ Department of Analytical Chemistry, School of Pharmaceutical Sciences, and COE Program in the 21st Century, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan.

PMID: 15997305
DOI: 10.1039/b505744b

Abstract

The resolution of asparagine-type oligosaccharides in glycoproteins was carried out by combination of the transglycosylation reaction and CE-TOF-MS. The oligosaccharides enzymatically transferred to a fluorescent acceptor (NDA-Asn-GlcNAc) with Endo-M. The resulting fluorescent-oligosaccharides were separated by CE and detected by TOF-MS. Disialo-Asn was successfully identified by the proposed procedure. Application to oligosaccharides in ovalbumin was also described in this communication.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylglucosamine / analogs & derivatives*
Acetylglucosamine / chemistry
Asparagine / analogs & derivatives*
Asparagine / chemistry
Electrophoresis, Capillary / methods*
Fluorescence
Glycopeptides / chemistry
Glycoproteins / chemistry*
Glycosylation
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / chemistry
Oligosaccharides / analysis*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Glycopeptides
Glycoproteins
Oligosaccharides
Asparagine
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Acetylglucosamine