Frequency of detection of picornaviruses and seven other respiratory pathogens in infants

Pediatr Infect Dis J. 2005 Jul;24(7):611-6. doi: 10.1097/01.inf.0000168747.94999.aa.


Background: Previous studies in which molecular-based techniques have been used to identify the causative pathogens of respiratory tract infection have investigated hospitalized children only. We report a prospective study designed to determine the frequency and clinical presentation of community-acquired respiratory illness in infancy associated with 8 common respiratory pathogens.

Methods: Eighty-eight infants were monitored through their first winter. With each respiratory illness, infants were examined, and a nasal lavage specimen was collected. Individual reverse transcription-polymerase chain reactions were performed to detect infection with picornaviruses (rhinoviruses and enteroviruses), coronaviruses (serotypes OC43 and 229E), adenoviruses, parainfluenza viruses 1-3, influenza viruses (types A and B), respiratory syncytial virus (RSV), Chlamydia pneumoniae and Mycoplasma pneumoniae.

Results: Picornaviruses were the most frequently detected pathogen identified in 46% (56 of 123) of episodes, followed by RSV (27%), parainfluenza viruses (13%) and coronaviruses (9%). Dual pathogen infections were identified in 20% of episodes, predominantly caused by picornaviruses together with either RSV or parainfluenza viruses. RSV infection was significantly associated with a diagnosis of bronchiolitis. No other associations were found between pathogen and clinical diagnosis. Dual infection did not predispose infants to a more severe clinical course.

Conclusions: Picornaviruses are the predominant cause of community-acquired respiratory tract infection in the first year of life. Large prospective community-based studies will be needed to fully evaluate the contribution of picornaviruses, both in isolation and in combination with other respiratory pathogens, to the various clinical syndromes of respiratory infection observed during infancy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Distribution
  • Chlamydophila Infections / epidemiology
  • Chlamydophila Infections / microbiology
  • Chlamydophila pneumoniae / isolation & purification*
  • Female
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Mycoplasma pneumoniae / isolation & purification*
  • Nasopharynx / microbiology
  • Picornaviridae / isolation & purification*
  • Picornaviridae Infections / epidemiology
  • Picornaviridae Infections / virology
  • Pneumonia, Mycoplasma / epidemiology
  • Pneumonia, Mycoplasma / microbiology
  • Respiratory Tract Infections / epidemiology*
  • Respiratory Tract Infections / microbiology
  • Respiratory Tract Infections / virology
  • Seasons
  • Sex Distribution
  • Virus Diseases / epidemiology
  • Virus Diseases / virology
  • Viruses / classification
  • Viruses / isolation & purification*