Comparison of susceptibility of fungal isolates to lufenuron and nikkomycin Z alone or in combination with itraconazole

Am J Vet Res. 2005 Jun;66(6):1090-3. doi: 10.2460/ajvr.2005.66.1090.


Objective: To evaluate and compare the in vitro antifungal properties of lufenuron and nikkomycin Z against isolates of Coccidioides immitis and Aspergillus fumigatus when used singly and in combination with the azole antifungal agent itraconazole.

Sample population: 3 clinical isolates of A fumigatus and the Silveira strain of C immitis.

Procedure: The fungal isolates were tested in vitro for susceptibility to the single and combination of compounds by use of microtiter-format susceptibility methods. Minimum inhibitory concentration end points were determined visually, and the contents of representative wells were examined microscopically for evidence of morphologic effects on fungi.

Results: No evidence of inhibition, either by susceptibility testing or direct microscopic examination of treated cells, was obtained with lufenuron under experimental conditions. In contrast, nikkomycin Z, a known inhibitor of fungal chitin synthesis, had potent activity against C immitis when used singly. A synergistic interaction between nikkomycin Z and itraconazole was found against isolates of both species tested.

Conclusions and clinical relevance: On the basis of our in vitro data, lufenuron does not appear to possess antifungal properties.

Publication types

  • Comparative Study

MeSH terms

  • Aminoglycosides / toxicity*
  • Antifungal Agents / toxicity*
  • Aspergillus fumigatus / drug effects*
  • Benzamides / toxicity*
  • Coccidioides / drug effects*
  • Itraconazole / toxicity*
  • Microbial Sensitivity Tests


  • Aminoglycosides
  • Antifungal Agents
  • Benzamides
  • fluphenacur
  • Itraconazole
  • nikkomycin