New Insights Into Metabolic Properties of Marine Bacteria Encoding Proteorhodopsins

PLoS Biol. 2005 Aug;3(8):e273. doi: 10.1371/journal.pbio.0030273. Epub 2005 Jul 19.

Abstract

Proteorhodopsin phototrophy was recently discovered in oceanic surface waters. In an effort to characterize uncultured proteorhodopsin-exploiting bacteria, large-insert bacterial artificial chromosome (BAC) libraries from the Mediterranean Sea and Red Sea were analyzed. Fifty-five BACs carried diverse proteorhodopsin genes, and we confirmed the function of five. We calculate that proteorhodopsin-exploiting bacteria account for 13% of microorganisms in the photic zone. We further show that some proteorhodopsin-containing bacteria possess a retinal biosynthetic pathway and a reverse sulfite reductase operon, employed by prokaryotes oxidizing sulfur compounds. Thus, these novel phototrophs are an unexpectedly large and metabolically diverse component of the marine microbial surface water.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / classification
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / physiology
  • Carotenoids / biosynthesis
  • Carotenoids / genetics
  • Chromosomes, Artificial, Bacterial / genetics
  • Cloning, Molecular
  • Conserved Sequence
  • Escherichia coli / genetics
  • Gene Library
  • Indian Ocean
  • Light
  • Mediterranean Sea
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Operon
  • Oxidoreductases Acting on Sulfur Group Donors / genetics
  • Phylogeny
  • Proteobacteria / classification
  • Proteobacteria / genetics*
  • Proteobacteria / metabolism
  • Rhodopsin / classification
  • Rhodopsin / genetics*
  • Rhodopsin / physiology
  • Rhodopsins, Microbial
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • Bacterial Proteins
  • Rhodopsins, Microbial
  • proteorhodopsin
  • Carotenoids
  • Rhodopsin
  • Oxidoreductases Acting on Sulfur Group Donors

Associated data

  • GENBANK/AY599897
  • GENBANK/AY599898
  • GENBANK/AY599899
  • GENBANK/AY599900
  • GENBANK/AY599901
  • GENBANK/AY599902
  • GENBANK/AY599903
  • GENBANK/AY599904
  • GENBANK/AY599905
  • GENBANK/AY599906
  • GENBANK/AY599907
  • GENBANK/AY599908
  • GENBANK/AY599909
  • GENBANK/CH024977
  • GENBANK/DQ062244
  • GENBANK/DQ062245
  • GENBANK/DQ062246
  • GENBANK/DQ062247
  • GENBANK/DQ062248
  • GENBANK/DQ062249
  • GENBANK/DQ062250
  • GENBANK/DQ062251
  • GENBANK/DQ062252
  • GENBANK/DQ062253
  • GENBANK/DQ062254
  • GENBANK/DQ062255
  • GENBANK/DQ062256
  • GENBANK/DQ062257
  • GENBANK/DQ062258
  • GENBANK/DQ062259
  • GENBANK/DQ062260
  • GENBANK/DQ062261
  • GENBANK/DQ062262
  • GENBANK/DQ062263
  • GENBANK/DQ062264
  • GENBANK/DQ062265
  • GENBANK/DQ062266
  • GENBANK/DQ062267
  • GENBANK/DQ062268
  • GENBANK/DQ062269
  • GENBANK/DQ062270
  • GENBANK/DQ062271
  • GENBANK/DQ062272
  • GENBANK/DQ062273
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  • GENBANK/DQ062275
  • GENBANK/DQ062276
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  • GENBANK/DQ062278
  • GENBANK/DQ062279
  • GENBANK/DQ062280
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  • GENBANK/DQ062293
  • GENBANK/DQ065755
  • GENBANK/DQ068067
  • GENBANK/DQ068068
  • GENBANK/DQ073796
  • GENBANK/DQ077553
  • GENBANK/DQ077554
  • GENBANK/DQ088847
  • GENBANK/DQ088848
  • GENBANK/DQ088849
  • GENBANK/DQ088850
  • GENBANK/DQ088851
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  • GENBANK/DQ088853
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  • GENBANK/DQ088855
  • GENBANK/DQ088856
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  • GENBANK/DQ088860
  • GENBANK/DQ088861
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  • GENBANK/DQ088871
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  • GENBANK/DQ088873
  • GENBANK/DQ088874
  • GENBANK/DQ088875
  • GENBANK/DQ088876
  • GENBANK/U84760
  • RefSeq/NC_000917
  • RefSeq/NC_002932