Effects of allogeneic bone marrow-derived mesenchymal stem cells on T and B lymphocytes from BXSB mice

DNA Cell Biol. 2005 Jul;24(7):458-63. doi: 10.1089/dna.2005.24.458.


Bone marrow-derived mesenchymal stem cells (bMSCs) can differentiate into a number of different cell/tissue types, and also possess immunoregulatory functions. The present study was undertaken to elucidate the exact immunoregulatory effects of allogeneic bMSCs on T- and B-lymphocyte proliferation, activation, and function maturation of BXSB mice, which has been considered as a experimental model for human systemic lupus erythematosus (SLE). We determined that bMSCs from BALB/c mice had inhibitory effects on BXSB mice T-lymphocyte proliferation, but no inhibitory effect on their activation. In addition, they had a significant inhibitory and stimulatory effect on IL-4- and IFN-gamma-producing T cells, respectively. Also, bMSCs had inhibitory effects on the proliferation, activation, and IgG secretion of B lymphocytes. In addition, BALB/c bMSCs had an enhancing effect on CD40 expression and inhibitory effects on CD40 ligand (CD40L) ectopic hyperexpression on B cells from BXSB mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / cytology
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology*
  • Bone Marrow Cells / cytology*
  • CD40 Antigens / metabolism
  • CD40 Ligand / metabolism
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Coculture Techniques
  • Concanavalin A / pharmacology
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Flow Cytometry
  • Genetic Markers / drug effects
  • Immunoglobulin G / metabolism
  • Interferon-gamma / metabolism
  • Interleukin-4 / metabolism
  • Lipopolysaccharides / pharmacology
  • Lupus Erythematosus, Systemic / immunology
  • Lymphocyte Activation / drug effects
  • Mesenchymal Stem Cells / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Mutant Strains
  • Mitogens / pharmacology
  • Spleen / cytology
  • T-Lymphocytes / cytology
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology*
  • Transplantation, Homologous / immunology*


  • CD40 Antigens
  • Genetic Markers
  • Immunoglobulin G
  • Lipopolysaccharides
  • Mitogens
  • Concanavalin A
  • CD40 Ligand
  • Interleukin-4
  • Interferon-gamma