Antigen Processing for Presentation by Class II Major Histocompatibility Complex Requires Cleavage by Cathepsin E

Eur J Immunol. 1992 Jun;22(6):1519-24. doi: 10.1002/eji.1830220626.

Abstract

Proteolytic degradation (processing) of antigen by antigen-presenting cells is a major regulatory step in the activation of a T lymphocyte immune response. However, the enzymes responsible for antigen processing remain largely undefined. In this study we show that cathepsin E, and not the ubiquitous lysosomal cathepsin D, is the major aspartic proteinase in a murine antigen-presenting cell line, A20. This enzyme is localized to a non-lysosomal compartment of the endosomal system in these cells. Functional studies using a highly specific inhibitor of cathepsin E show that this enzyme is essential for the processing of ovalbumin by this cell line. Thus, cathepsin E, whose function was hitherto unknown, may play a major role in antigen processing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen-Presenting Cells / physiology*
  • Aspartic Acid Endopeptidases / analysis
  • Aspartic Acid Endopeptidases / antagonists & inhibitors
  • B-Lymphocytes / immunology
  • Cathepsin E
  • Cathepsins / pharmacology*
  • Cell Fractionation
  • Chromatography, Gel
  • Dose-Response Relationship, Immunologic
  • Fluorescent Antibody Technique
  • Histocompatibility Antigens Class II / metabolism*
  • Interleukin-2 / metabolism
  • Macrophages / immunology
  • Mice
  • Mice, Inbred CBA
  • Molecular Weight
  • Ovalbumin / metabolism*
  • Pepstatins / pharmacology
  • T-Lymphocytes / immunology

Substances

  • Histocompatibility Antigens Class II
  • Interleukin-2
  • Pepstatins
  • Streptomyces pepsin inhibitor
  • Ovalbumin
  • Cathepsins
  • Aspartic Acid Endopeptidases
  • Cathepsin E
  • pepstatin